胚胎肺和正常肺组织基因表达谱差异

doi:10.3969/j.issn.1004-616x.2004.02.008

癌变·畸变·突变 ›› 2004, Vol. 16 ›› Issue (2): 92-93.doi: 10.3969/j.issn.1004-616x.2004.02.008

胚胎肺和正常肺组织基因表达谱差异

张　薇; 李殿俊;石玉枝

哈尔滨医科大学第一临床医学院呼吸内科，黑龙江　哈尔滨　150001

收稿日期:2003-10-20 修回日期:2004-02-16 出版日期:2004-03-30 发布日期:2004-03-30
通讯作者: 张　薇

The Difference of Gene Expression Profile in Fetal Lung and Normal Tissue

ZHANG Wei;LI Dian-jun;SHI Yu-zhi

Department of respiratory medicine, The First Affiliated Hospital of Harbin Medical University

Received:2003-10-20 Revised:2004-02-16 Online:2004-03-30 Published:2004-03-30
Contact: ZHANG Wei

摘要/Abstract

摘要： 背景与目的：利用基因芯片技术研究胚胎肺和正常肺组织基因表达谱差异，筛选出人肺在发育过程中基因的变化。材料与方法：分别抽取胚胎肺组织和正常肺组织的总RNA并纯化mRNA，用逆转录的方法，将各mRNA的两种荧光cy5和cy3标记的cDNA链做探针，并与含有1 152条人类全长基因芯片上进行杂交。以ScanArray4000荧光扫描仪扫描芯片上两种荧光信号，用计算机处理和分析。结果：胚胎肺和正常肺组织比较差异2倍以上基因共有317条，其中193条表达增加，124条表达降低。结论：胚胎肺和正常肺组织之间存在有基因差异，初步证明人肺组织在发育过程中受环境影响。

关键词: 胚胎肺, 正常肺组织, 基因表达谱

Abstract: BACKGROUND ＆ AIM: To investigate the difference of gene expression in fetal lung and normal tissue. MATERIAL AND METHODS: The cDNA retro－transcribed from the mRNA which was purified from the total RNA extracted from fetal lung tissue and normal lung tissue were labeled with Cy5 and Cy3 fluorescence as the probes. Then we hybrided the probes hybrided were with the whole gene chip which was later scanned by ScanArray4000 fluorescence scanner to acquire the signal image and analyzed with software. RESULTS: 317 genes expressed in fetal lung and normal tissue were screened, among these genes,up-and-down-regulated genes were 193 and 124 respectively. CONCLUSION : There is difference in gene between fetal two Lung and normal tissues. Human Lung development is affected by environmental conditions.

Key words: fetal lung, normal tissue, gene expression profile

张　薇, 李殿俊, 石玉枝. 胚胎肺和正常肺组织基因表达谱差异[J]. 癌变·畸变·突变, 2004, 16(2): 92-93.

ZHANG Wei, LI Dian-jun, SHI Yu-zhi. The Difference of Gene Expression Profile in Fetal Lung and Normal Tissue[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2004, 16(2): 92-93.

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胚胎肺和正常肺组织基因表达谱差异

The Difference of Gene Expression Profile in Fetal Lung and Normal Tissue

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